- This examination appeal concerns the inventive step of the ‘method for imaging a biological sample’ of claim 1.
- D3 is the closest prior art and discloses a method for imaging a biological sample. “Document D3 fails to disclose the features relating to the corrective optics, i.e. that corrective optics are removably inserted between the detector and the flow cell [...] The technical effect of this difference is that spherical aberration is compensated for and the problem to be solved is therefore to improve image quality.”
- “Document D6 discloses a microscope for imaging different layers of a sample in a sandwich configuration. D6 further identifies a change in optical medium as the cause for aberration [...] and states that corrective optics are inserted to compensate spherical aberration due to a change in the optical medium [.] Therefore, corrective optics have been employed in document D6 for the same purpose as in claim 1. It would be obvious to the person skilled in the art, namely when the same result is to be achieved as in claim 1, to apply corrective optics with corresponding effect in the imaging method disclosed in document D3, thereby arriving at a method for imaging a biological sample according to claim 1.”
- “The appellant argued that D6 disclosed a specific type of microscope which recorded the track of cosmic rays. Thus, the technical fields of D6 and D3 differed significantly and the skilled person would not consult document D6 when starting from a sandwiched flow cell as disclosed in D3.”
- “The board is of the opinion that document D6 relates to optical microscopes in general (see title and column 1, lines 10 to 16: "Field of the Invention") and offers a solution (insertion of corrective optics) for the correction of spherical aberration that occurs due to imaging of layers at different levels (see claim 1, [...]). Therefore, the skilled person would consider document D6 and apply its teachings in order to solve the objective technical problem.”
- D6 is US 4,563,062.
- As a comment, I think that this is a case which illustrates that the credibility of the EPO depends on how good the technical members of the Board can assume the role of the notional skilled person. I don't know whether a skilled person working in the field of D3 (link) would consult D6. I note that the Board does not expressly identify the skilled person. I would say it is the skilled person working with the technology of D3. D3 is a patent application carefully drafted to not overly restrict the scope of application of the claimed device, though the focus of D3 appears to be DNA microarrays (‘Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome’; Wikipedia). So the skilled person would likely be an engineer involved in the design of microscopes for reading out such DNA microarrays. Perhaps this would be an optical engineer; perhaps it would be another kind of engineer. Similarly, D6 is a patent carefully stating the 'field of the invention' of the claimed optical system in the most general terms. In the very next paragraph, it acknowledges as prior art "an emulsion chamber is one of the known apparatus for recording the track of cosmic rays." At least prima facie, D6 does not seem to be the kind of handbook that illustrates basic principles of optical engineering in general. But perhaps an optical engineer would search broadly for any literature addressing the optical problem at issue, irrespective of the precise application (assuming that the skilled person of D3 is an optical engineer instead of a biologist).
EPO T 2069/17 - link
Reasons for the Decision
1. Main request - Inventive Step - Article 56 EPC
1.1 Closest prior art
Document D3 represents the closest prior art and discloses a method for imaging a biological sample, where the biological sample comprises a flow cell (100) with a first biological sample (21) disposed on a first surface (20) and a second biological sample (11) disposed on a second surface (10) of the flow cell, such that the first and second surfaces face each other (see paragraphs [0024] to [0026] and figures 1 and 3). The biological samples are excited by a radiation source and radiation emitted from the two surfaces is detected (see paragraphs [0012] "Fluoreszenzmessungen" and [0036] "Fluoreszenzdetektion"). According to D3, the fluorescence measurements are performed at the flow cell through the first surface (see paragraphs [0035]), i.e. the flow cell is mounted as a whole on an imaging station such that the first surface (20) is disposed between the detector and the second surface (10). This is confirmed by the statement that the measuring time is reduced due to fluorescence measurements at the first and second surfaces (see paragraph [0012]).